Search results for "454 Pyrosequencing"

showing 4 items of 4 documents

Bacterial communities in stratified humic lakes

2012

yhteisörakennelämpötilakerrostuneisuushiilihiilitasebacterial communityhumusjärvetbakteeriyhteisötgreenhouse gasnutrientshumic lakecarbon cycle454 pyrosequencingmetsäjärvetravinnepitoisuushiilipitoisuus
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METAGENOMIC ANALYSIS OF PHYTOPHTHORA DIVERSITY IN NURSERIES OF POTTED ORNAMENTAL SPECIES.

2015

The genetic diversity of Phytophthora was investigated in soil and root samples of potted ornamental and fruit tree plants collected in nurseries located in Apulia and Calabria (Southern Italy) using metagenomic approaches based on Phytophthora genus specific primers. PCR amplicons containing the ITS1 region of the rDNA were sequenced using both a conventional cloning and Sanger sequencing approach and a 454 pyrosequencing protocol. All sequences were accurately analyzed with an appropriate software and used as barcode for species identification utilizing a validated ITS database. The cloning/Sanger sequencing approach enabled the identification of nine different Phytophthora taxa (P. nicot…

Nurseries Metagenomic 454 pyrosequencing Phytophthora spp. genus specific primers.Settore AGR/12 - Patologia Vegetale
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A Metabarcoding Survey on the Fungal Microbiota Associated to the Olive Fruit Fly.

2017

The occurrence of interaction between insects and fungi is interesting from an ecological point of view, particularly when these interactions involve insect pests and plant pathogens within an agroecosystem. In this study, we aimed to perform an accurate analysis on the fungal microbiota associated to Bactrocera oleae (Rossi) through a metabarcoding approach based on 454 pyrosequencing. From this analysis, we retrieved 43,549 reads that clustered into 128 operational taxonomic units (OTUs), of which 29 resulted in the “core” associate fungi of B. oleae. This fungal community was mainly represented by sooty mould fungi, such as Cladosporium spp., Alternaria spp. and Aureobasidium spp., by pl…

0106 biological sciences0301 basic medicineBactrocera oleaeOlive fruit flySoil ScienceAureobasidium01 natural sciences03 medical and health sciencesMicrobial ecologyPseudocercospora454 PyrosequencingAscomycotaOleaBotanyColletotrichumAnimalsDNA Barcoding TaxonomicPseudocercosporaEcology Evolution Behavior and SystematicsEcological nicheHigh-throughput sequencingEcologybiologyBase SequenceEcologyfungiTephritidaefood and beveragesSettore AGR/12 - Patologia VegetaleAlternariaHigh-Throughput Nucleotide SequencingSequence Analysis DNAbiology.organism_classificationAlternaria030104 developmental biologySettore AGR/11 - Entomologia Generale E ApplicataColletotrichumDNA Intergenic454 Pyrosequencing; Bactrocera oleae; Cladosporium; Colletotrichum; High-throughput sequencing; PseudocercosporaCladosporium010606 plant biology & botanyCladosporiumMycobiomeMicrobial ecology
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De Novo Assembly and Functional Annotation of the Olive (Olea europaea) Transcriptome

2013

Olive breeding programmes are focused on selecting for traits as short juvenile period, plant architecture suited for mechanical harvest, or oil characteristics, including fatty acid composition, phenolic, and volatile compounds to suit new markets. Understanding the molecular basis of these characteristics and improving the efficiency of such breeding programmes require the development of genomic information and tools. However, despite its economic relevance, genomic information on olive or closely related species is still scarce. We have applied Sanger and 454 pyrosequencing technologies to generate close to 2 million reads from 12 cDNA libraries obtained from the Picual, Arbequina, and L…

Sanger sequencingSequence assemblyBreedingOleaDatabases GeneticBotanygenomicsGeneticsPlant OilsJuvenileCultivarOlea europaeaOlive OilMolecular BiologyGene LibraryExpressed Sequence TagsExpressed sequence tagbiologycDNA libraryMolecular Sequence AnnotationSequence Analysis DNAGeneral MedicineFull Papersbiology.organism_classificationMolecular Sequence AnnotationOleaFruitSeedsPyrosequencing454 pyrosequencingTranscriptomeGenome PlantDNA Research
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